TY - JOUR
T1 - Probiotic Intake Increases the Expression of Vitellogenin Genes in Laying Hens
AU - Mazanko, Maria S.
AU - Makarenko, Maksim S.
AU - Chistyakov, Vladimir A.
AU - Usatov, Alexander V.
AU - Prazdnova, Evgeniya V.
AU - Bren, Anzhelika B.
AU - Gorlov, Ivan F.
AU - Komarova, Zoya B.
AU - Weeks, Richard
AU - Chikindas, Michael L.
N1 - Publisher Copyright:
© 2019, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2019/12/1
Y1 - 2019/12/1
N2 - A promising approach for slowing down the rate of reproductive aging is the use of probiotic bacteria as a feed additive. In the current study was investigated the influence of the intake of a potential probiotic on the follicle content and expression of vitellogenin genes (vtg1, vtg2, vtg3) in aged hens. RNA was isolated from liver samples collected from 570-day-old laying hens and gene expression levels were measured using RT-PCR. Bacillus subtilis KATMIRA1933 supplementation had a positive effect on the number of formed follicles in hens and also triggered a significant increase in the relative expression levels of vtg1, vtg2, and vtg3. A Bacillus amyloliquefaciens B-1895 enriched diet or a combination of the two strains had a modest effect on both the number of follicles and the expression of vitellogenin genes. Additionally, the study demonstrates that vitellogenin mRNA expression levels can be considered as a biomarker in a convenient approach for analyzing the hen’s egg-laying ability.
AB - A promising approach for slowing down the rate of reproductive aging is the use of probiotic bacteria as a feed additive. In the current study was investigated the influence of the intake of a potential probiotic on the follicle content and expression of vitellogenin genes (vtg1, vtg2, vtg3) in aged hens. RNA was isolated from liver samples collected from 570-day-old laying hens and gene expression levels were measured using RT-PCR. Bacillus subtilis KATMIRA1933 supplementation had a positive effect on the number of formed follicles in hens and also triggered a significant increase in the relative expression levels of vtg1, vtg2, and vtg3. A Bacillus amyloliquefaciens B-1895 enriched diet or a combination of the two strains had a modest effect on both the number of follicles and the expression of vitellogenin genes. Additionally, the study demonstrates that vitellogenin mRNA expression levels can be considered as a biomarker in a convenient approach for analyzing the hen’s egg-laying ability.
KW - Bacillus
KW - Poultry
KW - Probiotic supplementation
KW - Reproductive aging
KW - Vitellogenin
KW - vtg
UR - https://www.scopus.com/pages/publications/85060673402
UR - https://www.scopus.com/pages/publications/85060673402#tab=citedBy
U2 - 10.1007/s12602-019-9519-y
DO - 10.1007/s12602-019-9519-y
M3 - Article
C2 - 30674007
AN - SCOPUS:85060673402
SN - 1867-1306
VL - 11
SP - 1324
EP - 1329
JO - Probiotics and Antimicrobial Proteins
JF - Probiotics and Antimicrobial Proteins
IS - 4
ER -