Procedures for Studying Binding of Interferon to Human Cells in Suspension Cultures

Jerome A. Langer, Sidney Pestka

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

This chapter describes a simple and rapid assay for the binding of interferon to cells in suspension culture. Although the method is applicable to the cells of any species, human cells are used in the examples provided. It is desirable to have a simple method for measuring the binding of interferon to cells which grow in suspension. Such cells include various naturally occurring circulating cells, both normal and malignant, including many which function in immune responses and for which interferon is a biological effector. There are also a large number of established human cell lines that grow in suspension. Many of these lines are derived from leukemic cells (e.g., Daudi, HL60, and KG-I) and may prove valuable in studying the various roles of interferon. Indeed, the Daudi lymphoblastoid cell line is probably the best characterized human cell line in terms of its interaction with interferon and has been useful in correlating the growth inhibition by interferon with its binding. Many suspension lines are relatively easy to grow in large quantities, making such cells attractive for work on the interferon receptor. The method described is rapid and convenient. Because the cells are sedimented through a sucrose cushion into an elongated tip, no additional washing of the cells after pelleting is required.

Original languageEnglish (US)
Pages (from-to)305-311
Number of pages7
JournalMethods in enzymology
Volume119
Issue numberC
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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