Production of single-stranded DNA in mammalian cells by means of a bacterial retron

Oleg Mirochnitchenko, Sumiko Inouye, Masayori Inouye

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

msDNA-Ec67, a peculiar multicopy single-stranded DNA of a specific sequence was produced in NIH3T3 mouse cells. Retron-Ec67, a retroelement from Escherichia coli, was introduced under the T7 polymerase promoter and the non-translated 5'-region of the encephalomyocarditis virus. The construct was then transfected into the NIH3T3 cells constitutively producing T7 RNA polymerase. Forty-eight hours after transfection, msDNA-Ec67 was detected in the cells by means of Southern blot hybridization and reverse transcriptase extension assay. The potential use of bacterial retrons as a vector for single-stranded DNA production in mammalian cells is discussed.

Original languageEnglish (US)
Pages (from-to)2380-2383
Number of pages4
JournalJournal of Biological Chemistry
Volume269
Issue number4
StatePublished - Jan 28 1994

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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