Production of transgenic soybean lines expressing the bean pod mottle virus coat protein precursor gene

Kong Di, Virginia Purcell, Glenn B. Collins, Said A. Ghabrial

Research output: Contribution to journalArticlepeer-review

110 Scopus citations

Abstract

Soybean (Glycine max. Merrill. cv. Fayette) cotyledonary nodes were transformed with bean pod mottle virus (BPMV) coat protein precursor (CP-P) gene via Agrobacterium-mediated transformation. The transformation rate was low, and only five primary transformants derived from five different cotyledons were obtained from 400 original cotyledons. Southern blot hybridization verified the integration of the BPMV CP-P gene. Inheritance and expression of this gene in R1 plants were also demonstrated. About 30% of R2 plants derived from one transgenic lineshowed complete resistance to BPMV infection, as assessed by symptomatology and ELISA, suggesting that homozygous, but not hemizygous, plants exhibit the resistant phenotype.

Original languageEnglish (US)
Pages (from-to)746-750
Number of pages5
JournalPlant Cell Reports
Volume15
Issue number10
DOIs
StatePublished - 1996
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

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