Production of transgenic soybean lines expressing the bean pod mottle virus coat protein precursor gene

Rong Di; Virginia Purcell; Glenn B. Collins; Said A. Ghabrial

doi:10.1007/BF00232220

Production of transgenic soybean lines expressing the bean pod mottle virus coat protein precursor gene

Rong Di, Virginia Purcell, Glenn B. Collins, Said A. Ghabrial

Research output: Contribution to journal › Article › peer-review

113 Scopus citations

Abstract

Soybean (Glycine max. Merrill. cv. Fayette) cotyledonary nodes were transformed with bean pod mottle virus (BPMV) coat protein precursor (CP-P) gene via Agrobacterium-mediated transformation. The transformation rate was low, and only five primary transformants derived from five different cotyledons were obtained from 400 original cotyledons. Southern blot hybridization verified the integration of the BPMV CP-P gene. Inheritance and expression of this gene in R₁ plants were also demonstrated. About 30% of R₂ plants derived from one transgenic lineshowed complete resistance to BPMV infection, as assessed by symptomatology and ELISA, suggesting that homozygous, but not hemizygous, plants exhibit the resistant phenotype.

Original language	English (US)
Pages (from-to)	746-750
Number of pages	5
Journal	Plant Cell Reports
Volume	15
Issue number	10
DOIs	https://doi.org/10.1007/BF00232220
State	Published - 1996
Externally published	Yes

All Science Journal Classification (ASJC) codes

Agronomy and Crop Science
Plant Science

Access to Document

10.1007/BF00232220

Cite this

@article{9f48ba8bdc6c48d685beec81162fd9e2,

title = "Production of transgenic soybean lines expressing the bean pod mottle virus coat protein precursor gene",

abstract = "Soybean (Glycine max. Merrill. cv. Fayette) cotyledonary nodes were transformed with bean pod mottle virus (BPMV) coat protein precursor (CP-P) gene via Agrobacterium-mediated transformation. The transformation rate was low, and only five primary transformants derived from five different cotyledons were obtained from 400 original cotyledons. Southern blot hybridization verified the integration of the BPMV CP-P gene. Inheritance and expression of this gene in R1 plants were also demonstrated. About 30% of R2 plants derived from one transgenic lineshowed complete resistance to BPMV infection, as assessed by symptomatology and ELISA, suggesting that homozygous, but not hemizygous, plants exhibit the resistant phenotype.",

author = "Rong Di and Virginia Purcell and Collins, {Glenn B.} and Ghabrial, {Said A.}",

year = "1996",

doi = "10.1007/BF00232220",

language = "English (US)",

volume = "15",

pages = "746--750",

journal = "Plant Cell Reports",

issn = "0721-7714",

publisher = "Springer Verlag",

number = "10",

}

TY - JOUR

T1 - Production of transgenic soybean lines expressing the bean pod mottle virus coat protein precursor gene

AU - Di, Rong

AU - Purcell, Virginia

AU - Collins, Glenn B.

AU - Ghabrial, Said A.

PY - 1996

Y1 - 1996

N2 - Soybean (Glycine max. Merrill. cv. Fayette) cotyledonary nodes were transformed with bean pod mottle virus (BPMV) coat protein precursor (CP-P) gene via Agrobacterium-mediated transformation. The transformation rate was low, and only five primary transformants derived from five different cotyledons were obtained from 400 original cotyledons. Southern blot hybridization verified the integration of the BPMV CP-P gene. Inheritance and expression of this gene in R1 plants were also demonstrated. About 30% of R2 plants derived from one transgenic lineshowed complete resistance to BPMV infection, as assessed by symptomatology and ELISA, suggesting that homozygous, but not hemizygous, plants exhibit the resistant phenotype.

AB - Soybean (Glycine max. Merrill. cv. Fayette) cotyledonary nodes were transformed with bean pod mottle virus (BPMV) coat protein precursor (CP-P) gene via Agrobacterium-mediated transformation. The transformation rate was low, and only five primary transformants derived from five different cotyledons were obtained from 400 original cotyledons. Southern blot hybridization verified the integration of the BPMV CP-P gene. Inheritance and expression of this gene in R1 plants were also demonstrated. About 30% of R2 plants derived from one transgenic lineshowed complete resistance to BPMV infection, as assessed by symptomatology and ELISA, suggesting that homozygous, but not hemizygous, plants exhibit the resistant phenotype.

UR - http://www.scopus.com/inward/record.url?scp=9444278452&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=9444278452&partnerID=8YFLogxK

U2 - 10.1007/BF00232220

DO - 10.1007/BF00232220

M3 - Article

AN - SCOPUS:9444278452

SN - 0721-7714

VL - 15

SP - 746

EP - 750

JO - Plant Cell Reports

JF - Plant Cell Reports

IS - 10

ER -

Production of transgenic soybean lines expressing the bean pod mottle virus coat protein precursor gene

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this