Promoter analysis of Helicobacter pylori genes with enhanced expression at low pH

Catherine C. McGowan, Antoaneta S. Necheva, Mark H. Forsyth, Timothy L. Cover, Martin J. Blaser

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


To identify Helicobacter pylori genes with expression that is enhanced under low pH conditions, we used subtractive hybridization methodology. We identified 28 acid-induced genes, of which 18 have known or putative functions. Six pairs of genes were co-transcribed. Primer extension analysis identified single or multiple transcriptional start points (tsp) for 14 of the 22 loci. Sequence analysis of the -10 regions upstream of the tsps revealed consensus motifs for multiple RNA polymerase sigma factors present in H. pylori (σ80, σ54 and σ28). No sequences resembling the -35 Escherichia coli consensus sequence (TTGACA) were present upstream of any of the genes. Both increased gene transcription and decreased mRNA decay contribute to the observed increase in H. pylori transcript abundance at acid pH. These studies document the complex response of H. pylori to environmental pH changes, and provide insight into mechanisms used for intragastric survival.

Original languageEnglish (US)
Pages (from-to)1225-1239
Number of pages15
JournalMolecular microbiology
Issue number5
StatePublished - Jun 2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology


Dive into the research topics of 'Promoter analysis of Helicobacter pylori genes with enhanced expression at low pH'. Together they form a unique fingerprint.

Cite this