Promoter variation and gene expression of mcr-1-harboring plasmids in clinical isolates of Escherichia coli and klebsiella pneumoniae from a Chinese Hospital

Bin Huang, Yuting He, Xingyan Ma, Renxin Cai, Jianming Zeng, Yang Lu, Weizheng Zhang, Kai Lan, E. Sunmei, Yi Wei Tang, Barry N. Kreiswirth, Cha Chen, Liang Chen

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Next-generation sequencing of 6 mcr-1-harboring Escherichia coli and Klebsiella pneumoniae isolates collected from a tertiary care hospital in China revealed significant sequence variations in the regions flanking the mcr-1 gene. While sequence variations significantly affected the expression and promoter activity of mcr-1, the mcr-1 gene expression levels did not correlate with the in vitro colistin resistance levels, which warrants further in-depth investigations.

Original languageEnglish (US)
Article numbere00018
JournalAntimicrobial agents and chemotherapy
Volume62
Issue number5
DOIs
StatePublished - May 2018

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Keywords

  • Colistin
  • Gene expression
  • Genomics
  • Plasmids
  • Promoter

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