Prophage induction in lysogenic Aggregatibacter actinomycetemcomitans cells co-cultured with human gingival fibroblasts, and its effect on leukotoxin release

Roy H. Stevens, Caroline de Moura Martins Lobo dos Santos, David Zuanazzi, Marcelo Barbosas de Accioly Mattos, Davis Fernandes Ferreira, Scott Kachlany, Eduardo M.B. Tinoco

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Lysogeny is common among strains of the periodontal pathogen Aggregatibacter actinomycetemcomitans. Since lysogenic induction is known to result in the increased synthesis and release of bacterial toxins from lysogens, it would be important to elucidate the conditions under which induction of these bacteria may occur. Co-cultures of A. actinomycetemcomitans strains (either lysogenic or non-lysogenic) and human cells (either gingival fibroblasts or pharyngeal epithelial cells) were prepared. Following incubation, bacteriophage titers of up to 6.2 × 107 pfu/ml were detected in the cell-free, spent culture media from the co-cultures of the lysogenic A. actinomycetemcomitans strains and the fibroblasts. Little (maximum of 2 × 100 pfu/ml) or no titers of phage could be detected in the mono-cultures of the lysogenic A. actinomycetemcomitans strains alone. In contrast, no phage were detectable in the cell-free spent culture media of the lysogens cocultured with the epithelial cells. Futhermore, co-culture of the A. actinomycetemcomitans lysogens with the fibroblasts resulted in enhanced release of the A. actinomycetemcomitans leukotoxin into the culture medium, in comparison with the spent culture media from mono-cultures of the lysogens alone. These results are consistent with the concept that interaction with fibroblasts may mediate prophage induction in lysogenic strains of A. actinomycetemcomitans, and that leukotoxin release is greatly augmented following induction of the lysogens.

Original languageEnglish (US)
Pages (from-to)54-59
Number of pages6
JournalMicrobial Pathogenesis
Volume54
Issue number1
DOIs
StatePublished - Jan 1 2013

Fingerprint

Aggregatibacter actinomycetemcomitans
Virus Activation
Cultured Cells
Fibroblasts
Culture Media
Coculture Techniques
Bacteriophages
Epithelial Cells
Lysogeny
Bacterial Toxins
leukotoxin
Bacteria

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Infectious Diseases

Keywords

  • Aggregatibacter actinomycetemcomitans
  • Bacteriophage
  • Induction
  • Leukotoxin
  • Lysogeny

Cite this

Stevens, Roy H. ; Moura Martins Lobo dos Santos, Caroline de ; Zuanazzi, David ; Accioly Mattos, Marcelo Barbosas de ; Ferreira, Davis Fernandes ; Kachlany, Scott ; Tinoco, Eduardo M.B. / Prophage induction in lysogenic Aggregatibacter actinomycetemcomitans cells co-cultured with human gingival fibroblasts, and its effect on leukotoxin release. In: Microbial Pathogenesis. 2013 ; Vol. 54, No. 1. pp. 54-59.
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abstract = "Lysogeny is common among strains of the periodontal pathogen Aggregatibacter actinomycetemcomitans. Since lysogenic induction is known to result in the increased synthesis and release of bacterial toxins from lysogens, it would be important to elucidate the conditions under which induction of these bacteria may occur. Co-cultures of A. actinomycetemcomitans strains (either lysogenic or non-lysogenic) and human cells (either gingival fibroblasts or pharyngeal epithelial cells) were prepared. Following incubation, bacteriophage titers of up to 6.2 × 107 pfu/ml were detected in the cell-free, spent culture media from the co-cultures of the lysogenic A. actinomycetemcomitans strains and the fibroblasts. Little (maximum of 2 × 100 pfu/ml) or no titers of phage could be detected in the mono-cultures of the lysogenic A. actinomycetemcomitans strains alone. In contrast, no phage were detectable in the cell-free spent culture media of the lysogens cocultured with the epithelial cells. Futhermore, co-culture of the A. actinomycetemcomitans lysogens with the fibroblasts resulted in enhanced release of the A. actinomycetemcomitans leukotoxin into the culture medium, in comparison with the spent culture media from mono-cultures of the lysogens alone. These results are consistent with the concept that interaction with fibroblasts may mediate prophage induction in lysogenic strains of A. actinomycetemcomitans, and that leukotoxin release is greatly augmented following induction of the lysogens.",
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Prophage induction in lysogenic Aggregatibacter actinomycetemcomitans cells co-cultured with human gingival fibroblasts, and its effect on leukotoxin release. / Stevens, Roy H.; Moura Martins Lobo dos Santos, Caroline de; Zuanazzi, David; Accioly Mattos, Marcelo Barbosas de; Ferreira, Davis Fernandes; Kachlany, Scott; Tinoco, Eduardo M.B.

In: Microbial Pathogenesis, Vol. 54, No. 1, 01.01.2013, p. 54-59.

Research output: Contribution to journalArticle

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AU - Stevens, Roy H.

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