Prostaglandin E2 receptor of rat submandibular salivary glands

C. Y. Wu-Wang, S. L. Wang, P. Yao, A. Slomiany, B. L. Slomiany

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13 Scopus citations

Abstract

The binding characteristics of the PGE2 receptor were investigated in membrane preparations from these glands. Specific [3H]PGE2 binding was linear as a function of the membrane protein concentration and reached steady state by 40 min of incubation at 37 °C under neutral pH. Scatchard analysis of the binding data produced a curvilinear plot with a Kd of 0.18 nM and Bmax of 1.02 fmol/mg protein for the high-affinity binding sites, and a Kd of 181 nM and Bmax of 5.72 pmol/mg protein for the low-affinity binding sites. A competitive displacement study indicated that the receptor was specific for prostaglandins of the E series. The study is the first to demonstrate the presence of the PGE2 receptor in rat submandibular gland and to provide its biochemical features.

Original languageEnglish (US)
Pages (from-to)637-640
Number of pages4
JournalArchives of Oral Biology
Volume36
Issue number9
DOIs
StatePublished - 1991

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

Keywords

  • prostaglandin E
  • receptors
  • submandibular salivary gland

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