Prostanoid synthesis in isolated parenchymal and nonparenchymal mouse liver cells in the presence of arachioonic acid

Zoltán Spolarics; István Mucha; József Mandl; Raymund Machovich; Gábor Bánhegyi; Ferenc Antonio; Tamáas Garzó

doi:10.1016/0262-1746(87)90001-1

Prostanoid synthesis in isolated parenchymal and nonparenchymal mouse liver cells in the presence of arachioonic acid

Zoltán Spolarics, István Mucha, József Mandl, Raymund Machovich, Gábor Bánhegyi, Ferenc Antonio, Tamáas Garzó

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Prostanoid synthesis was investigated in suspensions of isolated mouse hepatocytes and nonparenchymal liver cells. A stable metabolite of thromboxane A₂ (TXB₂) of prostacyclin (6-keto PGF_1∝) and one of the prostaglandins (PGF_2∝) was detected by radio-immuno-assay (RIA). Hepatocytes synthesized mainly TXB₂, while smaller amounts of 6-keto PGF_1∝ and PGF_2∝ were detected during 60 min incubation. Homogenization of hepatocytes caused a slight increase in TXB₂ production and provoked the synthesis of PGF_2∝ and 6-keto PGF_1∝. Theaddition ofarachidonate to hepatocytes did not influence prostanoid production at concentrations below 10^-5 M. Higher concentrations further increased TXB₂ production and also increased the synthesis of 6-keto PGF_1∝ and PGF_2∝. Nonparenchymal cells synthesized all the three types of prostanoids and homogenization of these cells did not result in a marked change. The addition of 10^-7-10^-5M arachidonate increased the TXB₂, 6-keto PGF_1∝ and PGF_2∝ synthesis in nonparenchymal cells. No further increase was found at higher concentrations.

Original language	English (US)
Pages (from-to)	113-127
Number of pages	15
Journal	Prostaglandins, Leukotrienes and Medicine
Volume	29
Issue number	2-3
DOIs	https://doi.org/10.1016/0262-1746(87)90001-1
State	Published - Oct 1987
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Physiology
Endocrinology

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@article{4a4b1d29031249e5b5dad4a557171214,

title = "Prostanoid synthesis in isolated parenchymal and nonparenchymal mouse liver cells in the presence of arachioonic acid",

abstract = "Prostanoid synthesis was investigated in suspensions of isolated mouse hepatocytes and nonparenchymal liver cells. A stable metabolite of thromboxane A2 (TXB2) of prostacyclin (6-keto PGF1∝) and one of the prostaglandins (PGF2∝) was detected by radio-immuno-assay (RIA). Hepatocytes synthesized mainly TXB2, while smaller amounts of 6-keto PGF1∝ and PGF2∝ were detected during 60 min incubation. Homogenization of hepatocytes caused a slight increase in TXB2 production and provoked the synthesis of PGF2∝ and 6-keto PGF1∝. Theaddition ofarachidonate to hepatocytes did not influence prostanoid production at concentrations below 10-5 M. Higher concentrations further increased TXB2 production and also increased the synthesis of 6-keto PGF1∝ and PGF2∝. Nonparenchymal cells synthesized all the three types of prostanoids and homogenization of these cells did not result in a marked change. The addition of 10-7-10-5M arachidonate increased the TXB2, 6-keto PGF1∝ and PGF2∝ synthesis in nonparenchymal cells. No further increase was found at higher concentrations.",

author = "Zolt{\'a}n Spolarics and Istv{\'a}n Mucha and J{\'o}zsef Mandl and Raymund Machovich and G{\'a}bor B{\'a}nhegyi and Ferenc Antonio and Tam{\'a}as Garz{\'o}",

note = "Funding Information: Thanks are due to Mrs. Gizella Ferencz and Ms. Jolan Fazekas technical assistance. This work was supported by the Hungarian Sciences (l-3-86-329).",

year = "1987",

month = oct,

doi = "10.1016/0262-1746(87)90001-1",

language = "English (US)",

volume = "29",

pages = "113--127",

journal = "Prostaglandins Leukotrienes and Essential Fatty Acids",

issn = "0952-3278",

publisher = "Churchill Livingstone",

number = "2-3",

}

Prostanoid synthesis in isolated parenchymal and nonparenchymal mouse liver cells in the presence of arachioonic acid. / Spolarics, Zoltán; Mucha, István; Mandl, József; Machovich, Raymund; Bánhegyi, Gábor; Antonio, Ferenc; Garzó, Tamáas.

In: Prostaglandins, Leukotrienes and Medicine, Vol. 29, No. 2-3, 10.1987, p. 113-127.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Prostanoid synthesis in isolated parenchymal and nonparenchymal mouse liver cells in the presence of arachioonic acid

AU - Spolarics, Zoltán

AU - Mucha, István

AU - Mandl, József

AU - Machovich, Raymund

AU - Bánhegyi, Gábor

AU - Antonio, Ferenc

AU - Garzó, Tamáas

N1 - Funding Information: Thanks are due to Mrs. Gizella Ferencz and Ms. Jolan Fazekas technical assistance. This work was supported by the Hungarian Sciences (l-3-86-329).

PY - 1987/10

Y1 - 1987/10

N2 - Prostanoid synthesis was investigated in suspensions of isolated mouse hepatocytes and nonparenchymal liver cells. A stable metabolite of thromboxane A2 (TXB2) of prostacyclin (6-keto PGF1∝) and one of the prostaglandins (PGF2∝) was detected by radio-immuno-assay (RIA). Hepatocytes synthesized mainly TXB2, while smaller amounts of 6-keto PGF1∝ and PGF2∝ were detected during 60 min incubation. Homogenization of hepatocytes caused a slight increase in TXB2 production and provoked the synthesis of PGF2∝ and 6-keto PGF1∝. Theaddition ofarachidonate to hepatocytes did not influence prostanoid production at concentrations below 10-5 M. Higher concentrations further increased TXB2 production and also increased the synthesis of 6-keto PGF1∝ and PGF2∝. Nonparenchymal cells synthesized all the three types of prostanoids and homogenization of these cells did not result in a marked change. The addition of 10-7-10-5M arachidonate increased the TXB2, 6-keto PGF1∝ and PGF2∝ synthesis in nonparenchymal cells. No further increase was found at higher concentrations.

AB - Prostanoid synthesis was investigated in suspensions of isolated mouse hepatocytes and nonparenchymal liver cells. A stable metabolite of thromboxane A2 (TXB2) of prostacyclin (6-keto PGF1∝) and one of the prostaglandins (PGF2∝) was detected by radio-immuno-assay (RIA). Hepatocytes synthesized mainly TXB2, while smaller amounts of 6-keto PGF1∝ and PGF2∝ were detected during 60 min incubation. Homogenization of hepatocytes caused a slight increase in TXB2 production and provoked the synthesis of PGF2∝ and 6-keto PGF1∝. Theaddition ofarachidonate to hepatocytes did not influence prostanoid production at concentrations below 10-5 M. Higher concentrations further increased TXB2 production and also increased the synthesis of 6-keto PGF1∝ and PGF2∝. Nonparenchymal cells synthesized all the three types of prostanoids and homogenization of these cells did not result in a marked change. The addition of 10-7-10-5M arachidonate increased the TXB2, 6-keto PGF1∝ and PGF2∝ synthesis in nonparenchymal cells. No further increase was found at higher concentrations.

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JO - Prostaglandins Leukotrienes and Essential Fatty Acids

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