Prostanoid synthesis was investigated in suspensions of isolated mouse hepatocytes and nonparenchymal liver cells. A stable metabolite of thromboxane A2 (TXB2) of prostacyclin (6-keto PGF1∝) and one of the prostaglandins (PGF2∝) was detected by radio-immuno-assay (RIA). Hepatocytes synthesized mainly TXB2, while smaller amounts of 6-keto PGF1∝ and PGF2∝ were detected during 60 min incubation. Homogenization of hepatocytes caused a slight increase in TXB2 production and provoked the synthesis of PGF2∝ and 6-keto PGF1∝. Theaddition ofarachidonate to hepatocytes did not influence prostanoid production at concentrations below 10-5 M. Higher concentrations further increased TXB2 production and also increased the synthesis of 6-keto PGF1∝ and PGF2∝. Nonparenchymal cells synthesized all the three types of prostanoids and homogenization of these cells did not result in a marked change. The addition of 10-7-10-5M arachidonate increased the TXB2, 6-keto PGF1∝ and PGF2∝ synthesis in nonparenchymal cells. No further increase was found at higher concentrations.
All Science Journal Classification (ASJC) codes