Protective effects of human lactoferrin during aggregatibacter actinomycetemcomitans-Induced bacteremia in lactoferrin-Deficient mice

S. K. Velusamy, R. Poojary, R. Ardeshna, Waad Alabdulmohsen, D. H. Fine, K. Velliyagounder

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Aggregatibacter actinomycetemcomitans, a periodontopathogen, has been associated with several systemic diseases. Herein, we report the protective effect of human lactoferrin (hLF) during A. actinomycetemcomitans bacteremia in lactoferrin knockout (LFKO-/-) mice. The prophylactic, concurrent, and therapeutic intravenous (i.v.) administrations of hLF significantly cleared the bacteria from blood and organs. Nevertheless, all modes of hLF administration significantly decreased the concentrations of serum proinflammatory cytokines, such as interferon gamma (IFN-γ), tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), IL-6, IL-10, and IL-12p70. Additionally, hLF administration significantly decreased hepatic and splenic proinflammatory cytokine expression levels compared to those in the non-hLF-treated group. Furthermore, administration of hLF decreased the serum C-reactive protein level, inducible nitric oxide synthase (iNOS) and myeloperoxidase (MPO) gene expression levels in liver and spleen. hLF treatment has also resulted in a 6-fold decrease in spleen weight with the migration of typical inflammatory cells in infected mice as a result of decreased inflammatory response. These results reveal that hLF protects against A. actinomycetemcomitans bacteremia, as indicated by rapid bacterial clearance and decreased host proinflammatory mediators.

Original languageEnglish (US)
Pages (from-to)397-404
Number of pages8
JournalAntimicrobial agents and chemotherapy
Volume58
Issue number1
DOIs
StatePublished - Jan 2014

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Fingerprint

Dive into the research topics of 'Protective effects of human lactoferrin during aggregatibacter actinomycetemcomitans-Induced bacteremia in lactoferrin-Deficient mice'. Together they form a unique fingerprint.

Cite this