Protein kinase I of Mycobacterium tuberculosis: Cellular localization and expression during infection of macrophage-like cells

Anubha Singh, Yogendra Singh, Richard Pine, Lanbo Shi, Ramesh Chandra, Karl Drlica

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Protein kinase I of Mycobacterium tuberculosis, which has an unusual amino acid composition in its catalytic loop, displayed autophosphorylation and transphosphorylation activity. Immunoblot analysis of sub-cellular fractions of M. tuberculosis, using anti-PknI antibodies raised in rabbits, showed that PknI localizes to the bacterial cytosol. In contrast, PknA was membrane-bound. Relative expression of pknI, when measured by combining molecular beacons and RT-PCR, decreased during infection of THP-1 human macrophages. Expression of pknA and pknB was upregulated during infection. Thus PknI represents a group of protein kinases that is distinct from the more extensively studied enzymes PknA and PknB.

Original languageEnglish (US)
Pages (from-to)28-33
Number of pages6
JournalTuberculosis
Volume86
Issue number1
DOIs
StatePublished - Jan 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

Keywords

  • Molecular beacons
  • Mycobacterium tuberculosis
  • Protein kinase A
  • Protein kinase I
  • Transcription

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