TY - JOUR
T1 - Protein-nucleic acid interactions and DNA conformation in a complex of human immunodeficiency virus type 1 reverse transcriptase with a double-stranded DNA template-primer
AU - Ding, Jianping
AU - Hughes, Stephen H.
AU - Arnold, Edward
PY - 1997
Y1 - 1997
N2 - The conformation of the DNA and the interactions of the nucleic acid with the protein in a complex of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) and a 19-mer/18-mer double-stranded DNA template-primer (dsDNA) are described. The structure of this HIV-1 RT complex with dsDNA serves as a useful paradigm for studying aspects of nucleotide polymerases such as catalysis, fidelity, drug inhibition, and drug resistance. The bound dsDNA has a bend of approximately 41° at the junction of an A-form region (first five base pairs near the polymerase active site) and a B-form region (the last nine base pairs toward the RNase H active site). The 41° bend occurs smoothly over the four base pairs between the A-form portion and the B-form portion in the vicinity of helices αH and α1 of the p66 thumb subdomain. The interactions between the dsDNA and protein primarily involve the sugar-phosphate backbone of the nucleic acid and structural elements of the palm, thumb, and RNase H of p66, and are not sequence specific. Amino acid residues from the polymerase active site region, including amino acid residues of the conserved Tyr-Met-Asp-Asp (YMDD) motif and the "primer grip," interact with 3′-terminal nucleotides of the primer strand and are involved in positioning the primer terminal nucleotide and its 3′-OH group at the polymerase active site. Amino acid residues of the "template grip" have close contacts with the template strand and aid in positioning the template strand near the polymerase active site. Helix αH of the p66 thumb is partly inserted into the minor groove of the dsDNA and helix αI is directly adjacent to the backbone of the template strand. Amino acid residues of β1′, αA', αB', and the loop containing His539 of the RNase H domain interact with the primer strand of the dsDNA.
AB - The conformation of the DNA and the interactions of the nucleic acid with the protein in a complex of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) and a 19-mer/18-mer double-stranded DNA template-primer (dsDNA) are described. The structure of this HIV-1 RT complex with dsDNA serves as a useful paradigm for studying aspects of nucleotide polymerases such as catalysis, fidelity, drug inhibition, and drug resistance. The bound dsDNA has a bend of approximately 41° at the junction of an A-form region (first five base pairs near the polymerase active site) and a B-form region (the last nine base pairs toward the RNase H active site). The 41° bend occurs smoothly over the four base pairs between the A-form portion and the B-form portion in the vicinity of helices αH and α1 of the p66 thumb subdomain. The interactions between the dsDNA and protein primarily involve the sugar-phosphate backbone of the nucleic acid and structural elements of the palm, thumb, and RNase H of p66, and are not sequence specific. Amino acid residues from the polymerase active site region, including amino acid residues of the conserved Tyr-Met-Asp-Asp (YMDD) motif and the "primer grip," interact with 3′-terminal nucleotides of the primer strand and are involved in positioning the primer terminal nucleotide and its 3′-OH group at the polymerase active site. Amino acid residues of the "template grip" have close contacts with the template strand and aid in positioning the template strand near the polymerase active site. Helix αH of the p66 thumb is partly inserted into the minor groove of the dsDNA and helix αI is directly adjacent to the backbone of the template strand. Amino acid residues of β1′, αA', αB', and the loop containing His539 of the RNase H domain interact with the primer strand of the dsDNA.
KW - AIDS
KW - DNA structure
KW - Polymerase structure
KW - Protein-dNA interaction
KW - X-ray crystallography
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U2 - 10.1002/(sici)1097-0282(1997)44:2<125::aid-bip2>3.0.co;2-x
DO - 10.1002/(sici)1097-0282(1997)44:2<125::aid-bip2>3.0.co;2-x
M3 - Article
C2 - 9354757
AN - SCOPUS:0030786006
SN - 0006-3525
VL - 44
SP - 125
EP - 138
JO - Biopolymers
JF - Biopolymers
IS - 2
ER -