Protein P4 of the bacteriophage φ6 procapsid has a nucleoside triphosphate-binding site with associated nucleoside triphosphate phosphohydrolase activity

P. Gottlieb, J. Strassman, L. Mindich

Research output: Contribution to journalComment/debate

39 Scopus citations

Abstract

Bacteriophage φ6 contains three segments of double-stranded RNA. The procapsid consists of proteins P1, P2, P4, and P7, which are encoded by the viral L segment. cDNA copies of this segment have been cloned into plasmids that direct the production of these proteins, which assemble into polyhedral procapsids. These procapsids are capable of packaging plus-sense φ6 RNA in the presence of nucleoside triphosphate and synthesizing the complementary minus strand to form double-stranded RNA. In this article, we report the presence of a nucleotide-binding site in protein P4. The viral procapsid and nucleocapsid exhibit a nucleoside triphosphate phosphohydrolase activity that converts nucleoside triphosphates into nucleoside diphosphates.

Original languageEnglish (US)
Pages (from-to)6220-6222
Number of pages3
JournalJournal of virology
Volume66
Issue number10
DOIs
StatePublished - 1992

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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