Protein structure determination by combining sparse NMR data with evolutionary couplings

Yuefeng Tang, Yuanpeng Janet Huang, Thomas A. Hopf, Chris Sander, Debora S. Marks, Gaetano T. Montelione

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

Accurate determination of protein structure by NMR spectroscopy is challenging for larger proteins, for which experimental data are often incomplete and ambiguous. Evolutionary sequence information together with advances in maximum entropy statistical methods provide a rich complementary source of structural constraints. We have developed a hybrid approach (evolutionary coupling-NMR spectroscopy; EC-NMR) combining sparse NMR data with evolutionary residue-residue couplings and demonstrate accurate structure determination for several proteins 6â '41 kDa in size.

Original languageEnglish (US)
Pages (from-to)751-754
Number of pages4
JournalNature Methods
Volume12
Issue number8
DOIs
StatePublished - Jul 30 2015

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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