Abstract
Many key cellular interactions involve mechanotransduction (MT), whereby forces applied by other cells or by the matrix lead to biochemical and morphological sequellae. Since MT may be a useful target of molecular therapies, I have developed a rapid assay for it: the flow cytomechanical assay (FCMA). The assay exposes suspended cells to graded hydrostatic challenges prior to their entry into a standard flow cytometer. Preliminary results on 7 cell types have shown that FCMA can measure key aspects of MT at a rate of 10,000 cells per minute. FCMA could be a useful screening tool for agents and materials that involve MT.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 324-326 |
| Number of pages | 3 |
| Journal | Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings |
| Volume | 1 |
| State | Published - 2002 |
| Event | Proceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States Duration: Oct 23 2002 → Oct 26 2002 |
All Science Journal Classification (ASJC) codes
- Signal Processing
- Biomedical Engineering
- Computer Vision and Pattern Recognition
- Health Informatics
Keywords
- Cell volume regulation
- Flow cytometry
- Mechanotransduction
- Tamoxifen