Pseudouridine modification in Caenorhabditis elegans spliceosomal snRNAs: Unique modifications are found in regions involved in snRNA-snRNA interactions

Jeffrey R. Patton, Richard W. Padgett

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background. Pseudouridine (Ψ) is an abundant modified nucleoside in RNA and a number of studies have shown that the presence of Ψ affects RNA structure and function. The positions of Ψ in spliceosomal small nuclear RNAs (snRNAs) have been determined for a number of species but not for the snRNAs from Caenorhabditis elegans (C. elegans), a popular experimental model system of development. Results. As a prelude to determining the function of or requirement for this modification in snRNAs, we have mapped the positions of Ψ in U1, U2, U4, U5, and U6 snRNAs from worms using a specific primer extension method. As with other species, C. elegans U2 snRNA has the greatest number of Ψ residues, with nine, located in the 5′ half of the U2 snRNA. U5 snRNA has three Ψs, in or near the loop of the large stem-loop that dominates the structure of this RNA. U6 and U1 snRNAs each have one Ψ, and two Ψ residues were found in U4 snRNA. Conclusions. The total number of Ψs found in the snRNAs of C. elegans is significantly higher than the minimal amount found in yeasts but it is lower than that seen in sequenced vertebrate snRNAs. When the actual sites of modification on C. elegans snRNAs are compared with other sequenced snRNAs most of the positions correspond to modifications found in other species. However, two of the positions modified on C. elegans snRNAs are unique, one at position 28 on U2 snRNA and one at position 62 on U4 snRNA. Both of these modifications are in regions of these snRNAs that interact with U6 snRNA either in the spliceosome or in the U4/U6 small nuclear ribonucleoprotein particle (snRNP) and the presence of Ψ may be involved in strengthening the intermolecular association of the snRNAs.

Original languageEnglish (US)
Article number20
JournalBMC Molecular Biology
Volume6
DOIs
StatePublished - Oct 19 2005

Fingerprint

Pseudouridine
Small Nuclear RNA
Caenorhabditis elegans
RNA
U4-U6 Small Nuclear Ribonucleoproteins
Small Nuclear Ribonucleoproteins
Spliceosomes
Nucleosides
Yeast
Vertebrates
Theoretical Models
Yeasts

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

@article{962a8476ac4f40fab5b3615fbdfb014c,
title = "Pseudouridine modification in Caenorhabditis elegans spliceosomal snRNAs: Unique modifications are found in regions involved in snRNA-snRNA interactions",
abstract = "Background. Pseudouridine (Ψ) is an abundant modified nucleoside in RNA and a number of studies have shown that the presence of Ψ affects RNA structure and function. The positions of Ψ in spliceosomal small nuclear RNAs (snRNAs) have been determined for a number of species but not for the snRNAs from Caenorhabditis elegans (C. elegans), a popular experimental model system of development. Results. As a prelude to determining the function of or requirement for this modification in snRNAs, we have mapped the positions of Ψ in U1, U2, U4, U5, and U6 snRNAs from worms using a specific primer extension method. As with other species, C. elegans U2 snRNA has the greatest number of Ψ residues, with nine, located in the 5′ half of the U2 snRNA. U5 snRNA has three Ψs, in or near the loop of the large stem-loop that dominates the structure of this RNA. U6 and U1 snRNAs each have one Ψ, and two Ψ residues were found in U4 snRNA. Conclusions. The total number of Ψs found in the snRNAs of C. elegans is significantly higher than the minimal amount found in yeasts but it is lower than that seen in sequenced vertebrate snRNAs. When the actual sites of modification on C. elegans snRNAs are compared with other sequenced snRNAs most of the positions correspond to modifications found in other species. However, two of the positions modified on C. elegans snRNAs are unique, one at position 28 on U2 snRNA and one at position 62 on U4 snRNA. Both of these modifications are in regions of these snRNAs that interact with U6 snRNA either in the spliceosome or in the U4/U6 small nuclear ribonucleoprotein particle (snRNP) and the presence of Ψ may be involved in strengthening the intermolecular association of the snRNAs.",
author = "Patton, {Jeffrey R.} and Padgett, {Richard W.}",
year = "2005",
month = "10",
day = "19",
doi = "10.1186/1471-2199-6-20",
language = "English (US)",
volume = "6",
journal = "BMC Molecular Biology",
issn = "1471-2199",
publisher = "BioMed Central",

}

TY - JOUR

T1 - Pseudouridine modification in Caenorhabditis elegans spliceosomal snRNAs

T2 - Unique modifications are found in regions involved in snRNA-snRNA interactions

AU - Patton, Jeffrey R.

AU - Padgett, Richard W.

PY - 2005/10/19

Y1 - 2005/10/19

N2 - Background. Pseudouridine (Ψ) is an abundant modified nucleoside in RNA and a number of studies have shown that the presence of Ψ affects RNA structure and function. The positions of Ψ in spliceosomal small nuclear RNAs (snRNAs) have been determined for a number of species but not for the snRNAs from Caenorhabditis elegans (C. elegans), a popular experimental model system of development. Results. As a prelude to determining the function of or requirement for this modification in snRNAs, we have mapped the positions of Ψ in U1, U2, U4, U5, and U6 snRNAs from worms using a specific primer extension method. As with other species, C. elegans U2 snRNA has the greatest number of Ψ residues, with nine, located in the 5′ half of the U2 snRNA. U5 snRNA has three Ψs, in or near the loop of the large stem-loop that dominates the structure of this RNA. U6 and U1 snRNAs each have one Ψ, and two Ψ residues were found in U4 snRNA. Conclusions. The total number of Ψs found in the snRNAs of C. elegans is significantly higher than the minimal amount found in yeasts but it is lower than that seen in sequenced vertebrate snRNAs. When the actual sites of modification on C. elegans snRNAs are compared with other sequenced snRNAs most of the positions correspond to modifications found in other species. However, two of the positions modified on C. elegans snRNAs are unique, one at position 28 on U2 snRNA and one at position 62 on U4 snRNA. Both of these modifications are in regions of these snRNAs that interact with U6 snRNA either in the spliceosome or in the U4/U6 small nuclear ribonucleoprotein particle (snRNP) and the presence of Ψ may be involved in strengthening the intermolecular association of the snRNAs.

AB - Background. Pseudouridine (Ψ) is an abundant modified nucleoside in RNA and a number of studies have shown that the presence of Ψ affects RNA structure and function. The positions of Ψ in spliceosomal small nuclear RNAs (snRNAs) have been determined for a number of species but not for the snRNAs from Caenorhabditis elegans (C. elegans), a popular experimental model system of development. Results. As a prelude to determining the function of or requirement for this modification in snRNAs, we have mapped the positions of Ψ in U1, U2, U4, U5, and U6 snRNAs from worms using a specific primer extension method. As with other species, C. elegans U2 snRNA has the greatest number of Ψ residues, with nine, located in the 5′ half of the U2 snRNA. U5 snRNA has three Ψs, in or near the loop of the large stem-loop that dominates the structure of this RNA. U6 and U1 snRNAs each have one Ψ, and two Ψ residues were found in U4 snRNA. Conclusions. The total number of Ψs found in the snRNAs of C. elegans is significantly higher than the minimal amount found in yeasts but it is lower than that seen in sequenced vertebrate snRNAs. When the actual sites of modification on C. elegans snRNAs are compared with other sequenced snRNAs most of the positions correspond to modifications found in other species. However, two of the positions modified on C. elegans snRNAs are unique, one at position 28 on U2 snRNA and one at position 62 on U4 snRNA. Both of these modifications are in regions of these snRNAs that interact with U6 snRNA either in the spliceosome or in the U4/U6 small nuclear ribonucleoprotein particle (snRNP) and the presence of Ψ may be involved in strengthening the intermolecular association of the snRNAs.

UR - http://www.scopus.com/inward/record.url?scp=27744603243&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27744603243&partnerID=8YFLogxK

U2 - 10.1186/1471-2199-6-20

DO - 10.1186/1471-2199-6-20

M3 - Article

C2 - 16236171

AN - SCOPUS:27744603243

VL - 6

JO - BMC Molecular Biology

JF - BMC Molecular Biology

SN - 1471-2199

M1 - 20

ER -