PTA1, an Essential Gene of Saccharomyces cerevisiae Affecting Pre-tRNA Processing

James O'Connor, Craig L. Peebles

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We have identified an essential Saccharomyces cerevisiae gene, PTA1, that affects pre-tRNA processing. PTA1 was initially defined by a UV-induced mutation, pta1-1, that causes the accumulation of all 10 end-trimmed, intron-containing pre-tRNAs and temperature-sensitive but osmotic-remedial growth. pta1-1 does not appear to be an allele of any other known gene affecting pre-tRNA processing. Extracts prepared from pta1-1 strains had normal pre-tRNA splicing endonuclease activity. pta1-1 was suppressed by the ochre suppressor tRNA gene SUP11, indicating that the pta1-1 mutation creates a termination codon within a protein reading frame. The PTA1 gene was isolated from a genomic library by complementation of the pta1-1 growth defect. Episome-borne PTA1 directs recombination to the pta1-1 locus. PTA1 has been mapped to the left arm of chromosome I near CDC24; the gene was sequenced and could encode a protein of 785 amino acids with a molecular weight of 88,417. No other protein sequences similar to that of the predicted PTA1 gene product have been identified within the EMBL or GenBank data base. Disruption of PTA1 near the carboxy terminus of the putative open reading frame was lethal. Possible functions of the PTA1 gene product are discussed.

Original languageEnglish (US)
Pages (from-to)3843-3856
Number of pages14
JournalMolecular and cellular biology
Volume12
Issue number9
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

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Essential Genes
RNA Precursors
Saccharomyces cerevisiae
Genes
Suppressor Genes
Reading Frames
Mutation
Proteins
Genomic Library
Terminator Codon
Nucleic Acid Databases
Growth
Transfer RNA
Introns
Genetic Recombination
Open Reading Frames
Plasmids
Chromosomes
Molecular Weight
Alleles

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

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PTA1, an Essential Gene of Saccharomyces cerevisiae Affecting Pre-tRNA Processing. / O'Connor, James; Peebles, Craig L.

In: Molecular and cellular biology, Vol. 12, No. 9, 01.01.1992, p. 3843-3856.

Research output: Contribution to journalArticle

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