Pterostilbene Ameliorates DSS-Induced Intestinal Epithelial Barrier Loss in Mice via Suppression of the NF-κB-Mediated MLCK-MLC Signaling Pathway

Juan Wang, Hui Zhao, Ke Lv, Wei Zhao, Ning Zhang, Fan Yang, Xiang Wen, Xiaohua Jiang, Jingrui Tian, Xinjuan Liu, Chi Tang Ho, Shiming Li

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

The integrity of the intestinal barrier is critical for homeostasis. In this study, we investigated the protective effect of pterostilbene (PTE) on the intestinal epithelium barrier. In vitro results of transepithelial electrical resistance (TEER) in Caco-2 cells indicated that PTE counteracted tumor necrosis factor α (TNFα)-induced barrier damage. In vivo PTE pretreatment markedly ameliorated intestinal barrier dysfunction induced by dextran sulfate sodium (DSS). Notably, intestinal epithelial tight junction (TJ) molecules were restored by PTE in mice exposed to DSS. The mechanism study revealed that PTE prevented myosin light-chain kinase (MLCK) from driving phosphorylation of MLC (p-MLC), which is crucial for maintaining intestinal TJ stability. Furthermore, PTE blunted translocation of NF-κB subunit p65 into the nucleus to downregulate MLCK expression and then to safeguard TJs and barrier integrity. These findings suggest that PTE protected the intestinal epithelial barrier through the NF-κB- MLCK/p-MLC signal pathway.

Original languageEnglish (US)
Pages (from-to)3871-3878
Number of pages8
JournalJournal of agricultural and food chemistry
Volume69
Issue number13
DOIs
StatePublished - Apr 7 2021

All Science Journal Classification (ASJC) codes

  • General Chemistry
  • General Agricultural and Biological Sciences

Keywords

  • epithelial barrier
  • intestine
  • myosin light-chain kinase
  • pterostilbene
  • tight junction

Fingerprint

Dive into the research topics of 'Pterostilbene Ameliorates DSS-Induced Intestinal Epithelial Barrier Loss in Mice via Suppression of the NF-κB-Mediated MLCK-MLC Signaling Pathway'. Together they form a unique fingerprint.

Cite this