Pulse NMR studies of water permeability in phosphatidylcholine vesicles containing general anesthetics

Howard S. Hochster, J. H. Prestegard

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9 Scopus citations

Abstract

Phosphatidylcholine was extracted from egg yolks and sonicated with n-alkyl alcohols to give vesicles. The magnetic environment of the external compartment was then modified by the addition of shift reagent (Co2+). This allowed determination of the average lifetime for exchange of water molecule protons by observing the relaxation rate dependence on the interval between pulses in a Carr-Purcell sequence. For vesicles containing n-hexanol as 33% of lipid, the average water molecule lifetime in the internal compartment was found to be substantially less than that for a vesicle without hexanol. The lifetime, 11.3±4 msec, is equivalent to a permeability coefficient of 60 μ/sec at 34°C.

Original languageEnglish (US)
Pages (from-to)303-307
Number of pages5
JournalThe Journal of Membrane Biology
Volume35
Issue number1
DOIs
StatePublished - Dec 1 1977
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Physiology
  • Cell Biology

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