Purification, crystallization and preliminary crystallographic studies of the complex of interferon-λ1 with its receptor

Eugenia Magracheva, Sergei Pletnev, Sergei Kotenko, Wei Li, Alexander Wlodawer, Alexander Zdanov

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Human interferon-λ1 (IFN-λ1Ins) and the extracellular domain of interferon-λ1 receptor (IFN-1R1) were expressed in Drosophila S2 cells and purified to homogeneity. Both IFN-λ1 Ins and interferon-λ1 produced from Escherichia coli (IFN-λ1Bac) were coupled with IFN-λ1R1 at room temperature and the complexes were purified by gel filtration. Both complexes were crystallized; the crystals were flash-frozen at 100 K and diffraction data were collected to 2.16 and 2.1 Å, respectively. Although the IFN-λ1Bac-IFN-λ1R1 and IFN-λ1 Ins-IFN-λ1R1 complexes differed only in the nature of the expression system used for the ligand, their crystallization conditions and crystal forms were quite different. A search for heavy-atom derivatives as well as molecular-replacement trials are in progress.

Original languageEnglish (US)
Pages (from-to)61-63
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume66
Issue number1
DOIs
StatePublished - Dec 25 2009

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

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