TY - JOUR
T1 - Purifying Low-Volume Combinatorial Polymer Libraries with Gel Filtration Columns
AU - Upadhya, Rahul
AU - Kanagala, Mythili J.
AU - Gormley, Adam J.
N1 - Funding Information:
R.U. and M.J.K. contributed equally to this work. R.U. acknowledges the support of the National Institute of General Medical Sciences of the National Institutes of Health under award number T32 GM008339. A.J.G. acknowledges financial support from the New Jersey Health Foundation.
Publisher Copyright:
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Recent advances in oxygen-tolerant controlled/living radical polymer chemistry now enable efficient synthesis of diverse and combinatorial polymer libraries. While library synthesis has been dramatically simplified, equally efficient purification strategies for removal of small-molecule impurities are not yet established in high throughput settings. It is shown that gel filtration columns for chromatography frequently used in the protein science community are well suited for high throughput polymer purification. Using either single-use columns or gel filtration plates, the purification of 32 diverse polymers is demonstrated in a library with >95% removal of small molecule impurities and >85% polymer retention in a single purification step. Doing so replaces the typical procedure of polymer precipitation, which requires solvent optimization for each polymer in a complex library. Overall, this work raises awareness in the polymer science community that gel filtration is amenable to purification of large polymer libraries and can speed up the progress of combinatorial polymer chemistry.
AB - Recent advances in oxygen-tolerant controlled/living radical polymer chemistry now enable efficient synthesis of diverse and combinatorial polymer libraries. While library synthesis has been dramatically simplified, equally efficient purification strategies for removal of small-molecule impurities are not yet established in high throughput settings. It is shown that gel filtration columns for chromatography frequently used in the protein science community are well suited for high throughput polymer purification. Using either single-use columns or gel filtration plates, the purification of 32 diverse polymers is demonstrated in a library with >95% removal of small molecule impurities and >85% polymer retention in a single purification step. Doing so replaces the typical procedure of polymer precipitation, which requires solvent optimization for each polymer in a complex library. Overall, this work raises awareness in the polymer science community that gel filtration is amenable to purification of large polymer libraries and can speed up the progress of combinatorial polymer chemistry.
KW - gel filtration columns
KW - low-volume combinatorial polymer libraries
KW - photoinduced electron/energy transfer-reversible addition-fragmentation chain-transfer
KW - polymer purification
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U2 - 10.1002/marc.201900528
DO - 10.1002/marc.201900528
M3 - Article
C2 - 31737977
AN - SCOPUS:85075205442
SN - 1022-1336
VL - 40
JO - Macromolecular Rapid Communications
JF - Macromolecular Rapid Communications
IS - 24
M1 - 1900528
ER -