Pyridoxal 5′ phosphate at concentrations < 0.5 mM inhibits polymerization of deoxynucleoside triphosphate catalysed by variety of DNA polymerases isolated from type C RNA tumor viruses, as well as E. coli, but does not affect the polymerase associated RNase H activity. Both phosphate and aldehyde groups of pyridoxal phosphate are essential for the inhibition which appears to be mediated through the reversible Schiff base.
|Original language||English (US)|
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jul 12 1976|
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology