Pyridoxal 5′ phosphate: A selective inhibitor of oncornaviral DNA polymerases

Mukund J. Modak

doi:10.1016/0006-291X(76)90266-7

Pyridoxal 5′ phosphate: A selective inhibitor of oncornaviral DNA polymerases

Mukund J. Modak

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Pyridoxal 5′ phosphate at concentrations < 0.5 mM inhibits polymerization of deoxynucleoside triphosphate catalysed by variety of DNA polymerases isolated from type C RNA tumor viruses, as well as E. coli, but does not affect the polymerase associated RNase H activity. Both phosphate and aldehyde groups of pyridoxal phosphate are essential for the inhibition which appears to be mediated through the reversible Schiff base.

Original language	English (US)
Pages (from-to)	180-187
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	71
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(76)90266-7
State	Published - Jul 12 1976
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(76)90266-7

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title = "Pyridoxal 5′ phosphate: A selective inhibitor of oncornaviral DNA polymerases",

abstract = "Pyridoxal 5′ phosphate at concentrations < 0.5 mM inhibits polymerization of deoxynucleoside triphosphate catalysed by variety of DNA polymerases isolated from type C RNA tumor viruses, as well as E. coli, but does not affect the polymerase associated RNase H activity. Both phosphate and aldehyde groups of pyridoxal phosphate are essential for the inhibition which appears to be mediated through the reversible Schiff base.",

author = "Modak, {Mukund J.}",

note = "Funding Information: This research is supported in part by NC1 grant CA 08748. I thank S. Smith for expert technical assistance and Drs. Marcus and Gomatos for general counsel. I also greatly acknowledge Drs. J. Gruber for SSV and RLV; J. Beard and M.A. Chirigos for AMV; W. Hardy for FeLV; and L. Loeb for r. coli DNA polymerase",

year = "1976",

month = jul,

day = "12",

doi = "10.1016/0006-291X(76)90266-7",

language = "English (US)",

volume = "71",

pages = "180--187",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

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T1 - Pyridoxal 5′ phosphate

T2 - A selective inhibitor of oncornaviral DNA polymerases

AU - Modak, Mukund J.

N1 - Funding Information: This research is supported in part by NC1 grant CA 08748. I thank S. Smith for expert technical assistance and Drs. Marcus and Gomatos for general counsel. I also greatly acknowledge Drs. J. Gruber for SSV and RLV; J. Beard and M.A. Chirigos for AMV; W. Hardy for FeLV; and L. Loeb for r. coli DNA polymerase

PY - 1976/7/12

Y1 - 1976/7/12

N2 - Pyridoxal 5′ phosphate at concentrations < 0.5 mM inhibits polymerization of deoxynucleoside triphosphate catalysed by variety of DNA polymerases isolated from type C RNA tumor viruses, as well as E. coli, but does not affect the polymerase associated RNase H activity. Both phosphate and aldehyde groups of pyridoxal phosphate are essential for the inhibition which appears to be mediated through the reversible Schiff base.

AB - Pyridoxal 5′ phosphate at concentrations < 0.5 mM inhibits polymerization of deoxynucleoside triphosphate catalysed by variety of DNA polymerases isolated from type C RNA tumor viruses, as well as E. coli, but does not affect the polymerase associated RNase H activity. Both phosphate and aldehyde groups of pyridoxal phosphate are essential for the inhibition which appears to be mediated through the reversible Schiff base.

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DO - 10.1016/0006-291X(76)90266-7

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AN - SCOPUS:0017073262

SN - 0006-291X

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SP - 180

EP - 187

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Pyridoxal 5′ phosphate: A selective inhibitor of oncornaviral DNA polymerases

Abstract

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