Quantitating adenylate nucleotides in diverse organisms

Michael J. Napolitano, Daniel H. Shain

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Quantitation of cellular adenylate levels (i.e., ATP, ADP, AMP) has widespread applications in physiological, metabolic and energetic studies. We have compared classical adenylate extraction procedures (i.e., perchloric acid, boiling) with a previously unreported proteinase K-based extraction technique. Our results suggest that all three techniques are comparable in soft animal tissue, but proteinase K-based extractions consistently generated higher adenylate yields from a broad range of organisms, particularly those containing a cell wall (e.g., alga, bacteria, fungi, plant).

Original languageEnglish (US)
Pages (from-to)69-77
Number of pages9
JournalJournal of Biochemical and Biophysical Methods
Volume63
Issue number1
DOIs
StatePublished - Apr 29 2005

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry

Keywords

  • ATP
  • Energy charge
  • Extraction
  • HPLC
  • Metabolite
  • Proteinase K

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