Quantitation of human immunodeficiency virus type 1 viral load in plasma using reverse transcriptase activity assay at a district hospital laboratory in Botswana: A decentralization pilot study

Madisa Mine, Keabetswe Bedi, Talkmore Maruta, Dignity Madziva, Modiri Tau, Tatenda Zana, Tendani Gaolathe, Sikhulile Moyo, Khumo Seipone, Ndwapi Ndwapi, Max Essex, Richard Marlink

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Roche COBAS Amplicor monitor version 1.5 assay is considered gold standard for viral load monitoring in Botswana. Due to its demand for elaborate infrastructure, viral load testing has been confined to the national HIV reference laboratories. Cavidi ExaVir Load version 2 assay was considered as a potential alternative to decentralize viral load testing to the rural/remote hospital laboratories and thus increase access to therapy. This study compared the performance of ExaVir Load v2 assay at a district hospital laboratory in Serowe and COBAS Amplicor monitor v1.5 assay at the Botswana Harvard HIV Reference Laboratory using quality assessment samples and plasma from HIV-positive individuals. ExaVir Load v2 and COBAS Amplicor monitor v1.5 assays had very good agreement; Kappa statistic 0.951. The COBAS Amplicor monitor v1.5 and ExaVir Load v2 assays detected HIV-1 RNA in 84 and 86 samples but did not detect HIV-1 RNA in 221 and 219 samples, respectively. The two assays detected HIV-1 RNA concordantly in 82 samples and were strongly correlated (r = 0.8554, P < 0.0001). ExaVir Load v2 assay provided a simple and reliable alternative viral load system that is adaptable to district hospital laboratories. The cost per test is less than RT-PCR. The ExaVir Load v2 systems have since been placed in four more district and primary hospital laboratories.

Original languageEnglish (US)
Pages (from-to)93-97
Number of pages5
JournalJournal of Virological Methods
Volume159
Issue number1
DOIs
StatePublished - Jul 1 2009

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All Science Journal Classification (ASJC) codes

  • Virology

Keywords

  • Cavidi ExaVir Load
  • HIV-1 RNA
  • HIV-1 RT
  • Resource limited
  • Roche COBAS Amplicor
  • Viral load

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