Quantitative liquid chromatographic technique for the simultaneous assay of tricyclic antidepressant drugs in plasma or serum

P. K. Sonsalla; T. A. Jennison; B. S. Finkle

doi:10.1093/clinchem/28.3.457

Quantitative liquid chromatographic technique for the simultaneous assay of tricyclic antidepressant drugs in plasma or serum

P. K. Sonsalla, T. A. Jennison, B. S. Finkle

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17 Scopus citations

Abstract

A quantitative 'high pressure' liquid-chromatographic assay for tricyclic antidepressant drugs in plasma or serum is described in which amitriptyline, 2 nortriptyline, imipramine, desipramine, doxepin, and desmethyldoxepin are separated with a 10 μm particle size silica column and a methanol/NH₄OH/NH₄NO₃ solvent system. The drugs and two internal standards are extracted with hexane/isoamyl alcohol, the solvent layer is evaporated at 40°C, and the drugs are detected at 254 nm. Drug concentrations are linear with absorbance from 25 to 1000 μg/L; within-assay and between-assay CVs are ≤ 10%.

Original language	English (US)
Pages (from-to)	457-461
Number of pages	5
Journal	Clinical Chemistry
Volume	28
Issue number	3
DOIs	https://doi.org/10.1093/clinchem/28.3.457
State	Published - 1982
Externally published	Yes

All Science Journal Classification (ASJC) codes

Clinical Biochemistry
Biochemistry, medical

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abstract = "A quantitative 'high pressure' liquid-chromatographic assay for tricyclic antidepressant drugs in plasma or serum is described in which amitriptyline, 2 nortriptyline, imipramine, desipramine, doxepin, and desmethyldoxepin are separated with a 10 μm particle size silica column and a methanol/NH4OH/NH4NO3 solvent system. The drugs and two internal standards are extracted with hexane/isoamyl alcohol, the solvent layer is evaporated at 40°C, and the drugs are detected at 254 nm. Drug concentrations are linear with absorbance from 25 to 1000 μg/L; within-assay and between-assay CVs are ≤ 10%.",

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year = "1982",

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AU - Jennison, T. A.

AU - Finkle, B. S.

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N2 - A quantitative 'high pressure' liquid-chromatographic assay for tricyclic antidepressant drugs in plasma or serum is described in which amitriptyline, 2 nortriptyline, imipramine, desipramine, doxepin, and desmethyldoxepin are separated with a 10 μm particle size silica column and a methanol/NH4OH/NH4NO3 solvent system. The drugs and two internal standards are extracted with hexane/isoamyl alcohol, the solvent layer is evaporated at 40°C, and the drugs are detected at 254 nm. Drug concentrations are linear with absorbance from 25 to 1000 μg/L; within-assay and between-assay CVs are ≤ 10%.

AB - A quantitative 'high pressure' liquid-chromatographic assay for tricyclic antidepressant drugs in plasma or serum is described in which amitriptyline, 2 nortriptyline, imipramine, desipramine, doxepin, and desmethyldoxepin are separated with a 10 μm particle size silica column and a methanol/NH4OH/NH4NO3 solvent system. The drugs and two internal standards are extracted with hexane/isoamyl alcohol, the solvent layer is evaporated at 40°C, and the drugs are detected at 254 nm. Drug concentrations are linear with absorbance from 25 to 1000 μg/L; within-assay and between-assay CVs are ≤ 10%.

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Quantitative liquid chromatographic technique for the simultaneous assay of tricyclic antidepressant drugs in plasma or serum

Abstract

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