Quantitative reactivity profiling predicts functional cysteines in proteomes

Eranthie Weerapana, Chu Wang, Gabriel M. Simon, Florian Richter, Sagar Khare, Myles B.D. Dillon, Daniel A. Bachovchin, Kerri Mowen, David Baker, Benjamin F. Cravatt

Research output: Contribution to journalArticlepeer-review

933 Scopus citations

Abstract

Cysteine is the most intrinsically nucleophilic amino acid in proteins, where its reactivity is tuned to perform diverse biochemical functions. The absence of a consensus sequence that defines functional cysteines in proteins has hindered their discovery and characterization. Here we describe a proteomics method to profile quantitatively the intrinsic reactivity of cysteine residues en masse directly in native biological systems. Hyper-reactivity was a rare feature among cysteines and it was found to specify a wide range of activities, including nucleophilic and reductive catalysis and sites of oxidative modification. Hyper-reactive cysteines were identified in several proteins of uncharacterized function, including a residue conserved across eukaryotic phylogeny that we show is required for yeast viability and is involved in iron-sulphur protein biogenesis. We also demonstrate that quantitative reactivity profiling can form the basis for screening and functional assignment of cysteines in computationally designed proteins, where it discriminated catalytically active from inactive cysteine hydrolase designs.

Original languageEnglish (US)
Pages (from-to)790-797
Number of pages8
JournalNature
Volume468
Issue number7325
DOIs
StatePublished - Dec 9 2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

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