Quick detection of FKS1 mutations responsible for clinical echinocandin resistance in Candida albicans

Catiana Dudiuk, Soledad Gamarra, Cristina Jimenez-Ortigosa, Florencia Leonardelli, Daiana Macedo, David S. Perlin, Guillermo Garcia-Effron

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

A rapid molecular-based assay for the detection of the Candida albicans FKS1 gene mutations responsible for resistance to echinocandin drugs was designed and evaluated. The assay consisted of a multiplexed PCR set of 5 tubes able to detect the most commonly described resistance mechanism, including FKS1 hot spot 1 and hot spot 2 mutations. The performance and specificity of the assay was evaluated using a double-blinded panel of 50 C. albicans strains. The assay showed a sensitivity of 96% and was able to detect all homozygous mutants included in the collection of strains, demonstrating that it is a robust, quick, and laborsaving method that is suitable for a routine clinical diagnostic laboratory.

Original languageEnglish (US)
Pages (from-to)2037-2041
Number of pages5
JournalJournal of clinical microbiology
Volume53
Issue number7
DOIs
StatePublished - Jul 1 2015

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Fingerprint

Dive into the research topics of 'Quick detection of FKS1 mutations responsible for clinical echinocandin resistance in Candida albicans'. Together they form a unique fingerprint.

Cite this