Rad23 links DNA repair to the ubiquitin/proteasome pathway

C. Schauber, L. Chen, P. Tongaonkar, I. Vega, D. Lambertson, W. Potts, K. Madura

Research output: Contribution to journalArticlepeer-review

413 Scopus citations

Abstract

Rad23 is an evolationarily conserved protein that is important for nucleotide excision repair. A regulatory role has been proposed for Rad23 because rad23 mutants are sensitive to ultraviolet light but are still capable of incising damaged DNA. Here we show that Rad23 interacts with the 26S proteasome through an amino-terminal ubiquitin-like domain (UbL(R23)). The carboxy terminus of Rad23 binds to the Rad4 DNA repair protein and creates a link between the DNA repair and proteasome pathways. The ultraviolet sensitivity caused by deletion of the UbL(R23) domain may therefore arise from its inability to interact with the proteasome. The fusion proteins glutathione S-transferase (GST)-Rad23 and Rad4-haemagglutinin (HA), and the proteasome subunits Cim3 and Cim5, cofractionate through consecutive chromatography steps. The ubiquitin-like domain of human Rad23 (UbL(R23)) also interacts with the human proteasome. These results demonstrate that ubiquitin-like domains (UbLs) represent a new class of proteasome-interacting motifs.

Original languageEnglish (US)
Pages (from-to)715-718
Number of pages4
JournalNature
Volume391
Issue number6668
DOIs
StatePublished - Feb 12 1998

All Science Journal Classification (ASJC) codes

  • General

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