Rapid method for preparation of pure veratryl alcohol for the assay of lignin peroxidase

Kanyawim Kirtikara, Karen M. Schaich, Julie M. Fagan, Chaim Frenkel

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

We present a simple method for the rapid preparation of veratryl alcohol by reducing commercially available veratraldehyde with sodium borohydride resulting in high-purity veratryl alcohol. The lag period in the activity of lignin peroxidase from Phanerochaete chrysosporium that is associated with use of commercial preparations of the substrate is eliminated with the use of pure veratryl alcohol. The compound affords also estimation of low enzymatic activity.

Original languageEnglish (US)
Pages (from-to)253-259
Number of pages7
JournalJournal of Microbiological Methods
Volume23
Issue number3
DOIs
StatePublished - Sep 1995

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

Keywords

  • 3,4-Dimethoxybenzyl alcohol
  • Lignin peroxidase assay
  • Phanerochaete chrysosporium
  • Veratryl alcohol

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