Rapid Processing and Drug Evaluation in Glioblastoma Patient-Derived Organoid Models with 4D Bioprinted Arrays

Michelle Chadwick, Chen Yang, Liqiong Liu, Christian Moya Gamboa, Kelly Jara, Howon Lee, Hatem E. Sabaawy

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Glioblastoma is the most common and deadly primary brain malignancy. Despite advances in precision medicine oncology (PMO) allowing the identification of molecular vulnerabilities in glioblastoma, treatment options remain limited, and molecular assays guided by genomic and expression profiling to inform patient enrollment in life-saving trials are lacking. Here, we generate four-dimensional (4D) cell-culture arrays for rapid assessment of drug responses in glioblastoma patient-derived models. The arrays are 3D printed with thermo-responsive shape memory polymer (SMP). Upon heating, the SMP arrays self-transform in time from 3D cell-culture inserts into histological cassettes. We assess the utility of these arrays with glioblastoma cells, gliospheres, and patient derived organoid-like (PDO) models and demonstrate their use with glioblastoma PDOs for assessing drug sensitivity, on-target activity, and synergy in drug combinations. When including genomic and drug testing assays, this platform is poised to offer rapid functional drug assessments for future selection of therapies in PMO.

Original languageEnglish (US)
Article number101365
JournaliScience
Volume23
Issue number8
DOIs
StatePublished - Aug 21 2020

All Science Journal Classification (ASJC) codes

  • General

Keywords

  • Cancer
  • Medical Biotechnology
  • Tissue Engineering

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