Rare-earth doped nanocomposites enable multiscale targeted short-wave infrared imaging of metastatic breast cancer

Mark C. Pierce, Laura M. Higgins, Vidya Ganapathy, Harini Kantamneni, Richard E. Riman, Charles M. Roth, Prabhas V. Moghe

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

We are investigating the ability of targeted rare earth (RE) doped nanocomposites to detect and track micrometastatic breast cancer lesions to distant sites in pre-clinical in vivo models. Functionalizing RE nanocomposites with AMD3100 promotes targeting to CXCR4, a recognized marker for highly metastatic disease. Mice were inoculated with SCP-28 (CXCR4 positive) and 4175 (CXCR4 negative) cell lines. Whole animal in vivo SWIR fluorescence imaging was performed after bioluminescence imaging confirmed tumor burden in the lungs. Line-scanning confocal fluorescence microscopy provided high-resolution imaging of RE nanocomposite uptake and native tissue autofluorescence in ex vivo lung specimens. Co-registered optical coherence tomography imaging allowed assessment of tissue microarchitecture. In conclusion, multiscale optical molecular imaging can be performed in pre-clinical models of metastatic breast cancer, using targeted RE-doped nanocomposites.

Original languageEnglish (US)
Title of host publicationDiagnosis and Treatment of Diseases in the Breast and Reproductive System
EditorsPaul J. Campagnola, Melissa C. Skala
PublisherSPIE
ISBN (Electronic)9781510605275
DOIs
StatePublished - 2017
EventDiagnosis and Treatment of Diseases in the Breast and Reproductive System - San Francisco, United States
Duration: Jan 28 2017Jan 29 2017

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10043
ISSN (Print)1605-7422

Other

OtherDiagnosis and Treatment of Diseases in the Breast and Reproductive System
Country/TerritoryUnited States
CitySan Francisco
Period1/28/171/29/17

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Keywords

  • Nanoparticles
  • SWIR imaging
  • infrared imaging
  • microscopy
  • pre-clinical imaging
  • upconversion

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