Rat adipose tissue amino acid metabolism in vivo as assessed by microdialysis and arteriovenous techniques

Timothy J. Kowalski, Guoyao Wu, Malcolm Watford

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


In fed, anesthetized rats, microdialysis demonstrated a net release of glycerol, glutamine, serine, tyrosine, and taurine and a net uptake of glutamate, aspartate, glycine, and arginine across the inguinal adipose depot. However, the results also indicated excessive proteolysis associated with implantation of the microdialysis probe, and a novel arteriovenous difference technique was developed. Arteriovenous difference across the inguinal fat pat demonstrated a net uptake of glucose and a net release of lactate and glycerol. Starvation (48 h) resulted in higher rates of glycerol and lactate release with lower rates of glucose uptake. A net uptake of triacylglycerol was seen in starved-refed animals. Net glutamine, tyrosine, and taurine release was seen in fed and starved animals, but in starved- refed animals taurine and serine were the only amine acids showing significant release. No significant net uptake or release of ammonia, pyruvate, or alanine was observed. These experiments confirm that adipose tissue is a site of glutamine synthesis and suggest that the principal substrates are derived from intracellular proteolysis. The results also demonstrate the viability of an arteriovenous difference technique for the study of adipose tissue in the rat.

Original languageEnglish (US)
Pages (from-to)E613-E622
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Issue number3 36-3
StatePublished - Sep 1997

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)


  • Adipose tissue blood flow
  • Branched chain amino acids
  • Glucose
  • Glutamine
  • Glycerol
  • Lactate


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