Rat liver regeneration following ablation with irreversible electroporation

Alexander Golberg, Bote G. Bruinsma, Maria Jaramillo, Martin L. Yarmush, Basak E. Uygun

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

During the past decade, irreversible electroporation (IRE) ablation has emerged as a promising tool for the treatment of multiple diseases including hepatic cancer. However, the mechanisms behind the tissue regeneration following IRE ablation have not been investigated. Our results indicate that IRE treatment immediately kills the cells at the treatment site preserving the extracellular architecture, in effect causing in vivo decellularization. Over the course of 4 weeks, progenitor cell differentiation, through YAP and notch pathways, together with hepatocyte expansion led to almost complete regeneration of the ablated liver leading to the formation of hepatocyte like cells at the ablated zone.We did not observe significant scarring or tumor formation at the regenerated areas 6 months post IRE. Our study suggests a new model to study the regeneration of liver when the naïve extracellular matrix is decellularized in vivo with completely preserved extracellular architecture.

Original languageEnglish (US)
Article numbere1571
JournalPeerJ
Volume2016
Issue number1
DOIs
StatePublished - 2016

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Keywords

  • Hepatocytes
  • Irreversible electroporation
  • Liver ablation
  • Liver regeneration
  • Progenitor cells
  • Pulsed electric fields
  • Scarless regeneration

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