TY - JOUR
T1 - Real-time PCR detection of dogwood anthracnose fungus in historical herbarium specimens from Asia
AU - Miller, Stephen
AU - Masuya, Hayato
AU - Zhang, Jian
AU - Walsh, Emily
AU - Zhang, Ning
N1 - Funding Information:
This work was supported by the Clark T. Rogerson Student Research Award of the Mycological Society of America to Miller and a startup fund to Zhang from Rutgers University.
Publisher Copyright:
© 2016 Miller et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2016/4
Y1 - 2016/4
N2 - Cornus species (dogwoods) are popular ornamental trees and important understory plants in natural forests of northern hemisphere. Dogwood anthracnose, one of the major diseases affecting the native North American Cornus species, such as C. Florida, is caused by the fungal pathogen Discula destructiva. The origin of this fungus is not known, but it is hypothesized that it was imported to North America with its host plants from Asia. In this study, a TaqMan real-time PCR assay was used to detect D. destructiva in dried herbarium and fresh Cornus samples. Several herbarium specimens from Japan and China were detected positive for D. destructiva, some of which were collected before the first report of the dogwood anthracnose in North America. Our findings further support that D. destructiva was introduced to North America from Asia where the fungus likely does not cause severe disease.
AB - Cornus species (dogwoods) are popular ornamental trees and important understory plants in natural forests of northern hemisphere. Dogwood anthracnose, one of the major diseases affecting the native North American Cornus species, such as C. Florida, is caused by the fungal pathogen Discula destructiva. The origin of this fungus is not known, but it is hypothesized that it was imported to North America with its host plants from Asia. In this study, a TaqMan real-time PCR assay was used to detect D. destructiva in dried herbarium and fresh Cornus samples. Several herbarium specimens from Japan and China were detected positive for D. destructiva, some of which were collected before the first report of the dogwood anthracnose in North America. Our findings further support that D. destructiva was introduced to North America from Asia where the fungus likely does not cause severe disease.
UR - http://www.scopus.com/inward/record.url?scp=84978997532&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84978997532&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0154030
DO - 10.1371/journal.pone.0154030
M3 - Article
C2 - 27096929
AN - SCOPUS:84978997532
SN - 1932-6203
VL - 11
JO - PloS one
JF - PloS one
IS - 4
M1 - e0154030
ER -