Recombinant bacterial RNA polymerase: Preparation and applications

Konstantin Kuznedelov; Konstantin Severinov

doi:10.1016/j.ymeth.2008.10.007

Recombinant bacterial RNA polymerase: Preparation and applications

Konstantin Kuznedelov, Konstantin Severinov

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Availability of DNA-dependent RNA polymerase from various bacteria is a key for setting up specific in vitro transcription systems necessary for understanding species-specific transcription regulation. We describe here two main strategies for recombinant RNA polymerase preparation-through in vitro reconstitution and heterologous co-overproduction in Escherichia coli. Both strategies can be used for preparation of large amounts of RNA polymerases from any bacteria for which sequences of rpo (RNA polymerase) genes are known.

Original language	English (US)
Pages (from-to)	44-52
Number of pages	9
Journal	Methods
Volume	47
Issue number	1
DOIs	https://doi.org/10.1016/j.ymeth.2008.10.007
State	Published - Jan 2009

All Science Journal Classification (ASJC) codes

Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)

Keywords

Aquifex aeolicus
Bacterial
Co-overexpression
In vitro reconstitution
RNA polymerase
Transcription

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10.1016/j.ymeth.2008.10.007

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title = "Recombinant bacterial RNA polymerase: Preparation and applications",

abstract = "Availability of DNA-dependent RNA polymerase from various bacteria is a key for setting up specific in vitro transcription systems necessary for understanding species-specific transcription regulation. We describe here two main strategies for recombinant RNA polymerase preparation-through in vitro reconstitution and heterologous co-overproduction in Escherichia coli. Both strategies can be used for preparation of large amounts of RNA polymerases from any bacteria for which sequences of rpo (RNA polymerase) genes are known.",

keywords = "Aquifex aeolicus, Bacterial, Co-overexpression, In vitro reconstitution, RNA polymerase, Transcription",

author = "Konstantin Kuznedelov and Konstantin Severinov",

note = "Funding Information: The project described above was supported by NIH RO1 Grants GM59295 and GM64503 and a Russian Academy of Sciences Presidium program grant in Cell and Molecular Biology to K.S.",

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AU - Kuznedelov, Konstantin

AU - Severinov, Konstantin

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N2 - Availability of DNA-dependent RNA polymerase from various bacteria is a key for setting up specific in vitro transcription systems necessary for understanding species-specific transcription regulation. We describe here two main strategies for recombinant RNA polymerase preparation-through in vitro reconstitution and heterologous co-overproduction in Escherichia coli. Both strategies can be used for preparation of large amounts of RNA polymerases from any bacteria for which sequences of rpo (RNA polymerase) genes are known.

AB - Availability of DNA-dependent RNA polymerase from various bacteria is a key for setting up specific in vitro transcription systems necessary for understanding species-specific transcription regulation. We describe here two main strategies for recombinant RNA polymerase preparation-through in vitro reconstitution and heterologous co-overproduction in Escherichia coli. Both strategies can be used for preparation of large amounts of RNA polymerases from any bacteria for which sequences of rpo (RNA polymerase) genes are known.

KW - Aquifex aeolicus

KW - Bacterial

KW - Co-overexpression

KW - In vitro reconstitution

KW - RNA polymerase

KW - Transcription

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JO - ImmunoMethods

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IS - 1

ER -

Recombinant bacterial RNA polymerase: Preparation and applications

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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