Recombinant bacterial RNA polymerase: Preparation and applications

Konstantin Kuznedelov, Konstantin Severinov

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Availability of DNA-dependent RNA polymerase from various bacteria is a key for setting up specific in vitro transcription systems necessary for understanding species-specific transcription regulation. We describe here two main strategies for recombinant RNA polymerase preparation-through in vitro reconstitution and heterologous co-overproduction in Escherichia coli. Both strategies can be used for preparation of large amounts of RNA polymerases from any bacteria for which sequences of rpo (RNA polymerase) genes are known.

Original languageEnglish (US)
Pages (from-to)44-52
Number of pages9
Issue number1
StatePublished - Jan 2009

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)


  • Aquifex aeolicus
  • Bacterial
  • Co-overexpression
  • In vitro reconstitution
  • RNA polymerase
  • Transcription


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