Recombinase-based reporter system and antisense technology to study gene expression and essentiality in hypoxic nonreplicating mycobacteria

Srinivasa P.S. Rao, Luis Camacho, Bee Huat Tan, Calvin Boon, David G. Russel, Thomas Dick, Kevin Pethe

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The study of the mechanisms used by Mycobacterium tuberculosis to survive in the absence of growth is hampered by the absence of appropriate genetic tools. Here, we report two strategies, a recombinase-based reporter system and an antisense technology, to study gene expression and essentiality in hypoxic nonreplicating mycobacteria. The recombinase-based reporter system relies on the resolution of an antibiotic marker flanked by the γδ-res sites. This system was developed to identify M. tuberculosis promoters, which are specifically expressed under anaerobic conditions. The antisense strategy was designed to study the role of a gene candidate during anaerobic survival. To validate this approach, the dosR, narK2 and rv2466c promoters were selected to drive dosR antisense mRNA expression in quiescent mycobacteria. The conditional knockout strains were found to be attenuated to adapt and survive under anaerobic conditions, as observed for the dosR knockout strain. Together, our work demonstrates that the recombinase-based reporter system and antisense technology represent two genetic tools useful for the identification and characterization of genes essential for the survival of hypoxic nonreplicating M. tuberculosis.

Original languageEnglish (US)
Pages (from-to)68-75
Number of pages8
JournalFEMS Microbiology Letters
Volume284
Issue number1
DOIs
StatePublished - Jul 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Keywords

  • Antisense
  • Hypoxic dormancy
  • Mycobacterium tuberculosis
  • Resolvase

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