Recovery of clinically important microorganisms from the BacT/Alert blood culture system does not require testing for seven days

Michael L. Wilson, Stanley Mirrett, L. Barth Reller, Melvin P. Weinstein, Larry G. Reimer

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

Recently, we published a comparison of the BacT/Alert blood culture system with the bactec 660/730 nonradiometric blood culture system using blood inocula of 5 ml per bottle. By reanalyzing data collected during that study, we found that, for true-positive isolates causing bacteremia or fungemia, 363 (97.6%) of 376 and 341 (97.7%) of 349 isolates were recovered by the end of day 5 of testing, and 364 (97.9%) of 376 and 343 (98.3%) of 349 isolates were recovered by the end of day 6 of testing for aerobic and anaerobic bottles, respectively. Most isolates recovered on days 6 (24 of 27) and 7 (20 of 25) of testing were either contaminants or indeterminate as a cause of sepsis. When used as recommended by the manufacturer, only six (1.3%) of 464 clinically important isolates recovered on test days 6-7 would have gone undetected had testing been limited to 5 days and four (0.9%) of 464 had testing been limited to 6 days. We conclude that BacT/Alert bottles can be tested for as few as 5 days and then discarded with minimal loss of true-positive isolates and maximal reduction of contaminants.

Original languageEnglish (US)
Pages (from-to)31-34
Number of pages4
JournalDiagnostic Microbiology and Infectious Disease
Volume16
Issue number1
DOIs
StatePublished - Jan 1993

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

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