Recovery, purification, and cloning of high-molecular-weight DNA from soil microorganisms

Mark R. Liles, Lynn L. Williamson, Jitsupang Rodbumrer, Vigdis Torsvik, Robert M. Goodman, Jo Handelsman

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

We describe here an improved method for isolating, purifying, and cloning DNA from diverse soil microbiota. Soil microorganisms were extracted from soils and embedded and lysed within an agarose plug. Nucleases that copurified with the metagenomic DNA were removed by incubating plugs with a high-salt and -formamide solution. This method was used to construct large-insert soil metagenomic libraries.

Original languageEnglish (US)
Pages (from-to)3302-3305
Number of pages4
JournalApplied and environmental microbiology
Volume74
Issue number10
DOIs
StatePublished - May 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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