Recruitment of Mec1 and Ddc1 checkpoint proteins to double-strand breaks through distinct mechanisms

T. Kondo, T. Wakayama, T. Naiki, K. Matsumoto, K. Sugimoto

Research output: Contribution to journalArticlepeer-review

227 Scopus citations


In response to DNA damage, eukaryotic cells activate checkpoint pathways that arrest cell cycle progression and induce the expression of genes required for DNA repair. In budding yeast, the homothallic switching (HO) endonuclease creates a site-specific double-strand break at the mating type (MAT) locus. Continuous HO expression results in the phosphorylation of Rad53, which is dependent on products of the ataxia telangiectasia mutated-related MEC1 gene and other checkpoint genes, including DDC1, RAD9, and RAD24. Chromatin immunoprecipitation experiments revealed that the Ddc1 protein associates with a region near the MAT locus after HO expression. Ddc1 association required Rad24 but not Mec1 or Rad9. Mec1 also associated with a region near the cleavage site after HO expression, but this association is independent of Ddc1, Rad9, and Rad24. Thus, Mec1 and Ddc1 are recruited independently to sites of DNA damage, suggesting the existence of two separate mechanisms involved in recognition of DNA damage.

Original languageEnglish (US)
Pages (from-to)867-870
Number of pages4
Issue number5543
StatePublished - Oct 26 2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General


Dive into the research topics of 'Recruitment of Mec1 and Ddc1 checkpoint proteins to double-strand breaks through distinct mechanisms'. Together they form a unique fingerprint.

Cite this