Reductive debromination by sponge-associated anaerobic bacteria coupled to carbon isotope fractionation

Isabel Horna-Gray, Nora A. Lopez, Ivonne Nijenhuis, Youngbeom Ahn, Hans H. Richnow, Max M. Häggblom

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Marine sponges contain diverse brominated compounds as secondary metabolites and the sponge habitat appears to enrich for a population of anaerobic dehalogenating bacteria. Hence, there is interest in understanding how these natural and anthropogenic compounds are degraded in the marine environment. Compound specific isotope analysis (CSIA) is a useful tool to monitor and to quantify the degradation and fate of aquatic pollutants. The objective of this study was to evaluate whether reductive dehalogenation of brominated phenols by sponge-associated bacteria, including Desulfoluna spongiiphila, can be monitored by CSIA. Debromination of 2,6-dibromophenol to phenol by sponge-associated cultures resulted in measurable stable carbon isotope fractionation. All sponge-associated cultures showed similar isotopic enrichment factors (ε). The ε values for two independent sponge-derived dehalogenating cultures were −3.1 ± 1.5‰, and −3.0 ± 0.3‰, and that of sponge associated sediment cultures −2.0 ± 0.3‰. Thus, we demonstrate that reductive debromination of 2,6-dibromophenol resulted in measurable carbon isotope fractionation and that CSIA can be used to assess reductive debromination and to monitor and estimate in vivo dehalogenation in a sponge animal.

Original languageEnglish (US)
Article number105093
JournalInternational Biodeterioration and Biodegradation
Volume155
DOIs
StatePublished - Nov 2020

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Biomaterials
  • Waste Management and Disposal

Keywords

  • Anaerobic
  • Bromophenol
  • Carbon isotope fractionation
  • Microbial reductive dehalogenation

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