Regeneration of olfactory receptor neurons following chemical lesion: Ttime course and enhancement with growth factor administration

Christopher Herzog, Timothy Otto

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Although it has been known for over 50 years that olfactory receptor neuron (ORN) neurogenesis and subsequent reinnervation of the olfactory bulb (OB) occurs following ORN injury, the precise intrinsic and extrinsic factors that regulate this dynamic process have not yet been fully identified. In the first of two experiments, we characterized the time course of anatomical recovery following zinc sulfate (ZnSO4) lesion of ORNs in adult male Sprague-Dawley rats. ZnSO4 produced a near complete deafferentation of OB within 3 days following intranasal administration. A time-dependent increase in ORN reinnervation of OB was observed following 10, 20, and 30 day recovery intervals. Given the evidence that bFGF, EGF, and TGF-α have mitogenic effects on ORNs in vitro, a second experiment examined the extent to which these growth factors (GFs) might enhance ORN regeneration and subsequent reinnervation of OB in vivo. Rats received intranasal infusions of ZnSO4 on day 0, followed by subcutaneous injections of either bFGF (5, 10, or 50 μg/kg), EGF (5, 10, or 50 μg/kg), or TGF-α (5 or 10 μg/kg) on days 3-6. Horseradish peroxidase (HRP) histochemistry of OB following a 10-day recovery period revealed a dose-related enhancement in reinnervation of OB for each of the three growth factors examined, with the greatest enhancement produced by TGF-α. These data suggest that GFs may regulate ORN mitogenesis in vivo in a way similar to that which has been characterized in vitro. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)155-161
Number of pages7
JournalBrain research
Volume849
Issue number1-2
DOIs
StatePublished - Dec 4 1999

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Keywords

  • Growth factor
  • Neurogenesis
  • Olfactory system
  • Rat
  • Regeneration
  • Zinc sulfate

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