Regioselective 2'-silylation of purine ribonucleosides for phosphoramidite RNA synthesis.

B. L. Gaffney, R. A. Jones

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

This unit describes high-yield procedures for protection of purine ribonucleosides based on a reaction that allows concomitant highly regioselective 2'-silylation and 3'-phosphitylation to give, in one step, monomers that are ready for H-phosphonate synthesis. For preparation of phosphoramidites, the H-phosphonate monoester is cleaved without silyl migration to give intermediates ready for phosphitylation by standard methods.

Original languageEnglish (US)
Pages (from-to)Unit 2.8
JournalCurrent protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.]
VolumeChapter 2
DOIs
StatePublished - Nov 2001

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Organic Chemistry

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