Regulation of exoprotein gene expression in Staphylococcus aureus by agr

P. Recsei, B. Kreiswirth, M. O'Reilly, P. Schlievert, A. Gruss, R. P. Novick

Research output: Contribution to journalArticlepeer-review

402 Scopus citations

Abstract

Insertion of the erythromycin-resistance transposon Tn 551 into the Staphylococcus aureus chromosome at a site which maps between the purB and ilv loci has a pleiotrophic effect on the production of a number of extracellular proteins. Production of alpha, beta and delta hemolysin, toxic shock syndrome toxin (TSST-1) and staphylokinase was depressed about fifty-fold while protein A production was elevated twenty-fold. Hybridization analysis showed that the defect in expression of TSST-1 and alpha hemolysin was at the transcriptional level. Inability of the mutant strain to express either a cloned TSST-1 gene or the chromosomal gene indicates that the transposon has inactivated a trans-active positive control element. This element has been designated agr for accessory gene regulator.

Original languageEnglish (US)
Pages (from-to)58-61
Number of pages4
JournalMgg Molecular & General Genetics
Volume202
Issue number1
DOIs
StatePublished - Jan 1986
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

Keywords

  • Exoproteins
  • Gene regulation
  • Pathogenicity
  • Pletotropism
  • S. aureus

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