Regulation of inter- and intramolecular interaction of RNA, DNA, and proteins by MLE

Hyangyee Oh, Andrew M. Parrott, Yongkyu Park, Chee Gun Lee

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations


Drosophila maleless (MLE) is a member of helicase superfamily 2 and functions as a dosage compensation factor essential for the development of male flies. This function provides a good opportunity to investigate diverse biochemical activities associated with MLE in the context of a defined in vivo pathway, i.e., the transcriptional activation of X-linked genes. We have shown for the first time that MLE catalyzes the unwinding of both DNA and RNA and that MLE helicase activity is essential for its in vivo function. Also, we have provided evidence that MLE stimulates the transcriptional activity of roX2 on the X chromosome. We have also found that MLE interacts with dsDNA, topoisomerase II, and nucleosome. This observation supports a current model of dosage compensation: transcriptional activation of X-linked genes is causally associated with conformational change in the male X chromosome, subsequent to the targeted association of the dosage compensation complex (DCC).

Original languageEnglish (US)
Title of host publicationHelicases
Subtitle of host publicationMethods and Protocols
Number of pages24
StatePublished - 2010

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics


  • Maleless (MLE)
  • dosage compensation
  • roX2
  • topoisomerase II


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