Regulation of Muscle Contraction: Binding of Troponin and Its Components to Actin and Tropomyosin

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Abstract

The binding of troponin components to actin and tropomyosin has been studied by cosedimentation with actin and affinity chromatography. It is shown that troponin binds to actin and tropomyosin in the presence and absence of calcium but the binding to actin is sensitive to ionic strength. Troponin‐I + C binds to actin‐tropomyosin in the absence of calcium but not to actin or tropomyosin alone. Troponin‐I binds to actin and the binding is improved in the presence of tropomyosin even though troponin‐I does not bind to tropomyosin alone. Troponin‐C does not bind to actin or tropomyosin. The results suggest that the binding of troponin by actin is influenced by tropomyosin. A model of regulation by troponin is proposed.

Original languageEnglish (US)
Pages (from-to)255-263
Number of pages9
JournalEuropean Journal of Biochemistry
Volume52
Issue number2
DOIs
StatePublished - Mar 1975
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry

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