Regulation of phosphatidylinositol 3-kinase signalling pathway in the process of herbimycin A-induced erythroid differentiation of erythroleukemia cell line K562

The changes in protein - protein interactions mediated by SH3 domain of the regulatory p85α. subunit of phosphatidylinositol 3-kinase (PI 3-kinase) in the course of herbimycin A-induced erythroid differentiation of the human erythroleukemia cell line K562 have been analyzed. Binding assay was performed in vitro using a recombinant form of SH3 domain of p85α, conjugated with glutathione-S-transferase. pTyr-containing 210, 116, 52 and 46 KDa proteins, binding of which are modulated in differentiated cells, were identified and binding dynamics was analysed. The obtained data on binding the specific pTyr-containing proteins with regulatory subunit of PI 3-kinase testify about the coordinated control of PI 3-kinase signalling pathway in the course of herbimycin A-induced K562 cells differentiation.