Relaxation kinetics of the helix-coil transition of a self-complementary ribo-oligonucleotide: A7U7

Kenneth J. Breslauer, Minou Bina-Stein

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21 Scopus citations

Abstract

Relaxation measurements on the kinetics of the double helix to coil transition for the self-complementary ribo-oligonucleotide A7U7 are reported over a concentration range of 6.9 μM to 19.6 μM in single strand in 1 M NaCl. The rate constants for helix formation are about 2 × 106 M-1 s-1 and decrease with increasing temperature yielding an activation enthalpy of -6 kcal mole. The rate constants for helix dissociation range from 3 to 250 s-1 and increase with increasing temperature yielding an activation enthalpy of +45 kcal mole. The kinetic data reported here for 1 M NaCl is compared with previously published results obtained at lower salt concentrations. These data are discussed in terms of the quantitative effect of ionic strength on the kinetics of helix-coil transitions in oligo- and polynucleotides.

Original languageEnglish (US)
Pages (from-to)211-216
Number of pages6
JournalBiophysical chemistry
Volume7
Issue number3
DOIs
StatePublished - Nov 1977

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Organic Chemistry

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