Relaxed rotational and scrunching changes in P266L mutant of T7 RNA polymerase reduce short abortive RNAs while delaying transition into elongation

Biochemistry, Genetics and Molecular Biology

• RNA 100%
• Proline 100%
• Leucine 100%
• T7 RNA Polymerase 100%
• Promoter Region 70%
• Transcription 20%
• Binding Domain 20%
• Transcription Initiation 20%
• Rigidity 20%
• Wild Type 10%
• N-Terminus 10%
• C-Terminus 10%
• RNA Synthesis 10%
• DNA RNA Hybridization 10%
• Guanosine Triphosphate 10%
• RNA Polymerase I 10%
• Initiation Complex 10%
• Enzyme Active Site 10%
• Enterobacteria Phage T7 10%

Keyphrases

• Proline 100%
• Leucine 100%
• T7 RNA Polymerase 100%
• Ligand-binding Domain 20%
• Transcription Initiation 20%
• Linker Region 20%
• Promoter Binding 20%
• Adverse Effects 10%
• RNA Synthesis 10%
• Polymerase mutants 10%
• Promoter Region 10%
• Initial Transcription 10%
• In Vitro Transcription 10%
• DNA-dependent RNA Polymerase 10%
• Catalytic Domain 10%
• Linker 10%
• DNA-RNA Hybrids 10%
• Bacteriophage T7 10%
• Universal Characteristic 10%
• Initiation Complex 10%
• Promoter Opening 10%
• DNA Scrunching 10%
• Efficient Promoter 10%
• Linker Flexibility 10%
• Conformational Rigidity 10%

Immunology and Microbiology

• Promoter Region 100%
• T7 RNA Polymerase 100%
• Transcription Initiation 28%
• Rigidity 28%
• Wild Type 14%
• Carboxy Terminal Sequence 14%
• RNA Polymerase 14%
• RNA Synthesis 14%
• Amino Terminal Sequence 14%
• DNA RNA Hybridization 14%
• Enzyme Active Site 14%
• Enterobacteria Phage T7 14%