Release of 3H‐gamma‐aminobutyric acid (GABA) by inhibitory neurons of the crayfish

William Craelius, Russell A. Fricke

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Inhibitory neurons innervating the muscle receptor organ (MRO) of crayfish were used to study the uptake and release of tritiated GABA. MROs that have been directly exposed to 3H GABA for 60–75 min release radioactivity during low‐frequency electrical stimulation. When ganglia containing the inhibitory cell bodies are exposed to 3H GABA, the isotope travels along a pathway unique to the inhibitory axon, at rates that range between 160 and 240 mm per day. Electrical stimulation of inhibitory axons whose cell bodies have been exposed to 3H GABA for 4–5 hr produces release of isotope from isolated MROs. Low calcium, high magnesium exposure prevents the stimulus‐dependent release of radioactivity. Thin layer chromatographic analyses indicate that GABA comprises at least a major fraction of the radioactivity collected from stimulated preparations. A number of unidentified radioactive compounds are usually present with GABA, and it is suggested that most of these are catabolites of GABA.

Original languageEnglish (US)
Pages (from-to)249-258
Number of pages10
JournalJournal of Neurobiology
Volume12
Issue number3
DOIs
StatePublished - May 1981
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Release of <sup>3</sup>H‐gamma‐aminobutyric acid (GABA) by inhibitory neurons of the crayfish'. Together they form a unique fingerprint.

Cite this