Replikativnyǐ kompleks virusa kleshchevogo éntsefalita. I. Vyiavleniebelka iadernoǐ fraktsii infitsirovannykh kletok, otvetstvennykh za initsiatsiiu sinteza RNK, s pomoshch'iu affinnogo mecheniia.

Translated title of the contribution: Replication complex of tick-borne encephalitis complex. I. Identification of a nuclear fraction protein responsible for the initiation of RNA synthesis using affinity labeling

O. V. Morozova, N. A. Beliavskaia, E. F. Zaǐchikov, E. A. Kvetkova, A. A. Mustaev, A. G. Pletnev

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The pig embryo kidney cells infected by tick-borne encephalitis virus were fractionated into nuclear-associated, cytoplasmic and membrane fractions. The main part of the virus replicase activity was associated with the nuclei. The replication complex is able to synthesize full-length viral RNAs in vitro. To identify proteins involved in the initiation of the replication at the late stages of the infection, the highly specific affinity labelling technique was used. It was shown that with aldehyde-containing derivatives of ATP, ADP and AMP and [alpha-32P]GTP the target of labelling is a polypeptide having molecular weight about 69 kDa. The same protein is immunostained with TBE virus specific antibodies after blotting onto nitrocellulose. The conclusion is made that nonstructural protein NS3 takes part in virus replication at the late stage of the infection.

Translated title of the contributionReplication complex of tick-borne encephalitis complex. I. Identification of a nuclear fraction protein responsible for the initiation of RNA synthesis using affinity labeling
Original languageRussian
Pages (from-to)492-500
Number of pages9
JournalBioorganicheskaia khimiia
Volume16
Issue number4
StatePublished - Apr 1 1990
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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